产品描述

The human VEGFR-1 ELISA Kit is used to quantitatively measure human VEGFR-1 concentrations in serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity, specificity, precision, recovery, linearity, and lot-to-lot consistency. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of VEGFR-1 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

VEGFR-1, also called Flt-1, is a high-affinity tyrosine kinase receptor for VEGFs, including VEGF-A, VEGF-B, and PIGF. Distinct from VEGFR-2, which participates both in physiological and pathological angiogenesis, VEGFR-1 in the adult is necessary only for pathological angiogenesis. VEGFR-1-VEGF plays an essential role in tumor-related angiogenesis, tissue infiltration, and metastasis formation. Furthermore, VEGFR-1 activation contributes to the recruitment of tumor-associated macrophages (TAMs), cancer immune escape, and stimulation of migration and extracellular matrix (ECM) invasion.

别名

EC 2.7.10.1 ELISA Kit; FLT 1 ELISA Kit; FLT ELISA Kit; Flt-1 ELISA Kit; FLT1 ELISA Kit; Fms like tyrosine kinase 1 ELISA Kit; Fms related tyrosine kinase 1 ELISA Kit; Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) ELISA Kit; Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor ELISA Kit; Fms-like tyrosine kinase 1 ELISA Kit; FRT ELISA Kit; Soluble VEGF receptor 1 14 ELISA Kit; Soluble VEGFR1 variant 2 ELISA Kit; Soluble VEGFR1 variant 21 ELISA Kit; Tyrosine protein kinase FRT ELISA Kit; Tyrosine protein kinase receptor FLT ELISA Kit; Tyrosine-protein kinase FRT ELISA Kit; Tyrosine-protein kinase receptor FLT ELISA Kit; Vascular endothelial growth factor receptor 1 ELISA Kit; Vascular endothelial growth factor vascular permeability factor receptor ELISA Kit; Vascular permeability factor receptor 1 ELISA Kit; Vascular permeability factor receptor ELISA Kit; VEGFR 1 ELISA Kit; VEGFR-1 ELISA Kit; VEGFR1 ELISA Kit; VGFR1_HUMAN ELISA Kit

缩写

FLT1

Uniprot No

P17948

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGFR-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		86
		Range %		81-93
1:2		Average %		99
		Range %		94-104
1:4		Average %		92
		Range %		88-97
1:8		Average %		85
		Range %		81-90

回收率

The recovery of human VEGFR-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		85-96
EDTA plasma (n=4)		96		92-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.486 2.698 2.592 2.460 5 1.685 1.667 1.676 1.544 2.5 0.986 0.997 0.992 0.860 1.25 0.600 0.612 0.606 0.474 0.625 0.396 0.376 0.386 0.254 0.312 0.282 0.299 0.291 0.159 0.156 0.190 0.176 0.183 0.051 0 0.131 0.133 0.132

货期

3-5 working days