产品描述
IFNG(Interferon gamma)是一种重要的免疫因子,由多种细胞类型分泌。它可以刺激免疫细胞的活化和增殖,并且可以调节免疫细胞的基因表达和活性。IFNG在维持细胞免疫、抗病毒和抗肿瘤等生物学过程中发挥重要作用。IFNG的含量水平受许多因素影响,如年龄、疾病状态等。
华美生物所提供的Chicken Interferon γ ,IFN-γ ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测鸡血清、血浆、组织匀浆样本中的IFNG,其灵敏度为3.125 pg/mL,检测范围为3.125 pg/mL-800 pg/mL。
别名
IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写
IFNG
Uniprot No
P49708
灵敏度
3.125 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
91-100 |
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| 1:2 |
Average % |
92 |
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| Range % |
88-97 |
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| 1:4 |
Average % |
102 |
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| Range % |
98-106 |
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| 1:8 |
Average % |
94 |
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| Range % |
90-98 |
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回收率
| The recovery of chicken IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
93-101 |
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| EDTA plasma (n=4) |
92 |
88-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 800 |
0.178 |
0.182 |
0.180 |
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| 200 |
0.245 |
0.233 |
0.239 |
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| 50 |
0.457 |
0.482 |
0.470 |
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| 12.5 |
0.848 |
0.817 |
0.833 |
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| 3.125 |
1.396 |
1.364 |
1.380 |
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| 0 |
2.237 |
2.242 |
2.240 |
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货期
3-5 working days