产品描述

This rat AST ELISA Kit is suitable for qualitatively determining AST concentrations in multiple biological fluids, including rat serum, plasma, and tissue homogenates in vitro. AST, also called GOT1, is an important transaminase enzyme in amino acid metabolism that catalyzes the conversion of aspartate and alpha-ketoglutarate to oxaloacetate and glutamate. AST is a part of the malate-aspartate shuttle in the myocardium and participates in gluconeogenesis in the liver and kidney, glyceroneogenesis in the adipose tissue, and the production of neurotransmitters and neuro-glial pathways in the brain. A high AST level in the body can be an indicator of liver disease and other health problems.

This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of rat AST in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-rat AST antibody. Biotin-labeled AST antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the AST in the rat samples. AST binds to the biotinylated anti-rat AST monoclonal antibody. And the biotin on the biotinylated anti-rat AST monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of AST in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

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These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml OD1 OD2 Average Corrected 200 2.094 2.068 2.081 1.918 100 1.276 1.310 1.293 1.130 50 0.838 0.784 0.811 0.648 25 0.521 0.539 0.530 0.367 12.5 0.353 0.334 0.344 0.181 6.25 0.300 0.288 0.294 0.131 3.12 0.241 0.237 0.239 0.076 0 0.164 0.162 0.163

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