产品描述

This Human HSPA5 ELISA Kit was designed for the quantitative measurement of Human HSPA5 protein in serum, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

别名

78 kDa glucose regulated protein ELISA Kit; 78 kDa glucose-regulated protein ELISA Kit; AL022860 ELISA Kit; AU019543 ELISA Kit; BIP ELISA Kit; D2Wsu141e ELISA Kit; D2Wsu17e ELISA Kit; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 ELISA Kit; Endoplasmic reticulum lumenal Ca2+ binding protein grp78 ELISA Kit; Epididymis secretory sperm binding protein Li 89n ELISA Kit; FLJ26106 ELISA Kit; Glucose Regulated Protein 78 ELISA Kit; Glucose Regulated Protein 78kDa ELISA Kit; GRP 78 ELISA Kit; GRP-78 ELISA Kit; GRP78 ELISA Kit; GRP78_HUMAN ELISA Kit; Heat shock 70 kDa protein 5 ELISA Kit; Heat Shock 70kDa Protein 5 ELISA Kit; Heat shock protein family A (Hsp70) member 5 ELISA Kit; HEL S 89n ELISA Kit; Hsce70 ELISA Kit; HSPA 5 ELISA Kit; HSPA5 ELISA Kit; Immunoglobulin Heavy Chain Binding Protein ELISA Kit; Immunoglobulin heavy chain-binding protein ELISA Kit; mBiP ELISA Kit; MIF2 ELISA Kit; Sez7 ELISA Kit

缩写

HSPA5

Uniprot No

P11021

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human BIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		87-100
1:2		Average %		89
		Range %		84-95
1:4		Average %		85
		Range %		81-90
1:8		Average %		96
		Range %		92-101

回收率

The recovery of human BIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		83-96
EDTA plasma (n=4)		96		90-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.752 2.789 2.771 2.648 5 2.161 2.198 2.180 2.057 2.5 1.636 1.616 1.626 1.503 1.25 1.068 1.034 1.051 0.928 0.625 0.652 0.612 0.632 0.509 0.312 0.437 0.447 0.442 0.319 0.156 0.341 0.369 0.355 0.232 0 0.121 0.124 0.123

货期

3-5 working days