产品描述
This Rat IGF1R ELISA Kit was designed for the quantitative measurement of Rat IGF1R protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.8 pg/mL.
别名
Igf1r; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IGF-I receptor; CD antigen CD221
缩写
IGF1R
Uniprot No
P24062
灵敏度
0.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IGF-1R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
85 |
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| Range % |
81-89 |
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| 1:200 |
Average % |
92 |
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| Range % |
87-98 |
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| 1:400 |
Average % |
100 |
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| Range % |
97-103 |
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| 1:800 |
Average % |
96 |
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| Range % |
91-99 |
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回收率
| The recovery of rat IGF-1R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
110 |
107-113 |
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| EDTA plasma (n=4) |
89 |
85-93 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.406 |
2.461 |
2.434 |
2.278 |
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| 100 |
1.913 |
1.946 |
1.930 |
1.774 |
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| 50 |
1.526 |
1.534 |
1.530 |
1.374 |
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| 25 |
1.009 |
1.068 |
1.039 |
0.883 |
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| 12.5 |
0.684 |
0.698 |
0.691 |
0.535 |
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| 6.25 |
0.366 |
0.368 |
0.367 |
0.211 |
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| 3.12 |
0.257 |
0.271 |
0.264 |
0.108 |
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| 0 |
0.154 |
0.157 |
0.156 |
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货期
3-5 working days