产品描述
The human keratinocyte growth factor (KGF) ELISA Kit is engineered for accurate measurement of human KGF levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the KGF content in the sample. The color intensity is positively correlated with KGF content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.
KGF, also called FGF7, is a mesenchyme-specific heparin-binding growth factor that stimulates cell proliferation, differentiation, migration, and vascular angiogenesis. FGF7 is required for the development of the kidney, thymus, and hippocampus. It exerts its functions by binding to high-affinity receptor FGFR2IIIb. FGF7 is also involved in the healing process, which makes it an agent for reducing inflammation and injury. Several studies have shown that FGF7 is expressed in many types of cancer, including prostatic, breast, colorectal, and lung, and is associated with the proliferation and regulation of tumor cells.
别名
FGF 7 ELISA Kit; FGF-7 ELISA Kit; Fgf7 ELISA Kit; FGF7_HUMAN ELISA Kit; Fibroblast growth factor 7 ELISA Kit; HBGF 7 ELISA Kit; HBGF-7 ELISA Kit; HBGF7 ELISA Kit; Heparin binding growth factor 7 ELISA Kit; Heparin-binding growth factor 7 ELISA Kit; Keratinocyte growth factor ELISA Kit; KGF ELISA Kit
缩写
FGF7
Uniprot No
P21781
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
97 |
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| Range % |
93-102 |
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| 1:4 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:8 |
Average % |
92 |
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| Range % |
87-96 |
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回收率
| The recovery of human KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
93-101 |
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| EDTA plasma (n=4) |
86 |
81-92 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.413 |
2.542 |
2.478 |
2.362 |
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| 1000 |
1.941 |
1.848 |
1.895 |
1.779 |
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| 500 |
1.212 |
1.235 |
1.224 |
1.108 |
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| 250 |
0.764 |
0.752 |
0.758 |
0.642 |
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| 125 |
0.441 |
0.456 |
0.449 |
0.333 |
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| 62.5 |
0.297 |
0.284 |
0.291 |
0.175 |
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| 31.25 |
0.187 |
0.181 |
0.184 |
0.068 |
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| 0 |
0.115 |
0.116 |
0.116 |
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货期
3-5 working days
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