产品描述

This Human DDIT4 ELISA Kit was designed for the quantitative measurement of Human DDIT4 protein in serum, tissue homogenates, cell lysates, urine, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

别名

DDIT4 ELISA Kit; DDIT4_HUMAN ELISA Kit; Dig2 ELISA Kit; DNA damage inducible transcript 4 ELISA Kit; DNA damage inducible transcript 4 protein ELISA Kit; DNA damage-inducible transcript 4 protein ELISA Kit; FLJ20500 ELISA Kit; HIF 1 responsive protein RTP801 ELISA Kit; HIF 1 responsive RTP801 ELISA Kit; HIF-1 responsive protein RTP801 ELISA Kit; Protein regulated in development and DNA damage response 1 ELISA Kit; REDD-1 ELISA Kit; REDD1 ELISA Kit; RTP801 ELISA Kit

缩写

DDIT4

Uniprot No

Q9NX09

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human DDIT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		86-92
1:2		Average %		94
		Range %		89-98
1:4		Average %		96
		Range %		92-99
1:8		Average %		98
		Range %		92-103

回收率

The recovery of human DDIT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.948 2.754 2.851 2.691 5 2.539 2.412 2.476 2.316 2.5 1.781 1.662 1.722 1.562 1.25 1.248 1.198 1.223 1.063 0.625 0.738 0.706 0.722 0.562 0.312 0.514 0.504 0.509 0.349 0.156 0.305 0.298 0.302 0.142 0 0.162 0.158 0.160

货期

3-5 working days