产品描述
EGFR(epidermal growth factor receptor)是一种表皮生长因子受体,属于酪氨酸激酶受体家族。它能够诱导细胞增殖、分化和生存,并参与多种生理过程的调节。EGFR的异常表达与多种疾病的发生和发展密切相关,如在肿瘤、炎症性疾病、心血管疾病等中表达水平升高或降低。
华美生物所提供的Human epidermal growth factor receptor,EGFR ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的EGFR,其灵敏度为0.019 ng/mL,检测范围为0.312 ng/mL-20 ng/mL。
别名
Avian erythroblastic leukemia viral (v erb b) oncogene homolog ELISA Kit; Cell growth inhibiting protein 40 ELISA Kit; Cell proliferation inducing protein 61 ELISA Kit; EGF R ELISA Kit; EGFR ELISA Kit; EGFR_HUMAN ELISA Kit; Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) ELISA Kit; Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) ELISA Kit; Epidermal growth factor receptor ELISA Kit; erb-b2 receptor tyrosine kinase 1 ELISA Kit; ERBB ELISA Kit; ERBB1 ELISA Kit; Errp ELISA Kit; HER1 ELISA Kit; mENA ELISA Kit; NISBD2 ELISA Kit; Oncogen ERBB ELISA Kit; PIG61 ELISA Kit; Proto-oncogene c-ErbB-1 ELISA Kit; Receptor tyrosine protein kinase ErbB 1 ELISA Kit; Receptor tyrosine-protein kinase ErbB-1 ELISA Kit; SA7 ELISA Kit; Species antigen 7 ELISA Kit; Urogastrone ELISA Kit; v-erb-b Avian erythroblastic leukemia viral oncogen homolog ELISA Kit; wa2 ELISA Kit; Wa5 ELISA Kit
缩写
EGFR
Uniprot No
P00533
灵敏度
0.019 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
| |
|
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human EGFR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| |
Sample |
Serum(n=4) |
|
|
| 1:5 |
Average % |
101 |
|
|
| Range % |
95-104 |
|
|
| 1:10 |
Average % |
99 |
|
|
| Range % |
91-103 |
|
|
| 1:20 |
Average % |
94 |
|
|
| Range % |
87-98 |
|
|
| 1:40 |
Average % |
108 |
|
|
| Range % |
97-112 |
|
|
回收率
| The recovery of human EGFR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| |
| Sample Type |
Average % Recovery |
Range |
|
|
| Serum (n=5) |
103 |
96-107 |
|
|
| EDTA plasma (n=4) |
93 |
84-97 |
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
|
| 20 |
2.522 |
2.463 |
2.493 |
2.375 |
|
|
| 10 |
2.018 |
1.977 |
1.998 |
1.880 |
|
|
| 5 |
1.408 |
1.463 |
1.436 |
1.318 |
|
|
| 2.5 |
1.051 |
1.025 |
1.038 |
0.920 |
|
|
| 1.25 |
0.666 |
0.647 |
0.657 |
0.539 |
|
|
| 0.625 |
0.427 |
0.403 |
0.415 |
0.297 |
|
|
| 0.312 |
0.264 |
0.261 |
0.263 |
0.145 |
|
|
| 0 |
0.119 |
0.117 |
0.118 |
|
|
|
|
货期
3-5 working days