产品描述

This Human ESR1 ELISA Kit was designed for the quantitative measurement of Human ESR1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

别名

7*/654 isoform ELISA Kit; 7*/819 2 isoform ELISA Kit; 7*/822 isoform ELISA Kit; 8*/901 isoform ELISA Kit; 8*/941 isoform ELISA Kit; DKFZp686N23123 ELISA Kit; ER alpha ELISA Kit; ER ELISA Kit; ER-alpha ELISA Kit; Era ELISA Kit; ESR ELISA Kit; ESR1 ELISA Kit; ESR1_HUMAN ELISA Kit; ESRA ELISA Kit; Estradiol receptor ELISA Kit; Estrogen nuclear receptor alpha ELISA Kit; Estrogen receptor 1 ELISA Kit; Estrogen receptor alpha 3*,4,5,6,7*/822 isoform ELISA Kit; Estrogen receptor alpha ELISA Kit; Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform ELISA Kit; Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform ELISA Kit; Estrogen receptor alpha delta 4 +49 isoform ELISA Kit; Estrogen receptor alpha delta 4*,5,6,7*/654 isoform ELISA Kit; Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform ELISA Kit; Estrogen receptor alpha E1 E2 1 2 ELISA Kit; Estrogen receptor alpha E1 N2 E2 1 2 ELISA Kit; Estrogen receptor ELISA Kit; ESTRR ELISA Kit; NR3A1 ELISA Kit; Nuclear receptor subfamily 3 group A member 1 ELISA Kit

缩写

ESR1

Uniprot No

P03372

灵敏度

0.78 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		88-95
1:2		Average %		95
		Range %		90-100
1:4		Average %		96
		Range %		90-102
1:8		Average %		101
		Range %		96-106

回收率

The recovery of human ER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		87-95
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected     200 2.634 2.647 2.641 2.451     100 1.935 1.877 1.906 1.716     50 1.142 1.236 1.189 0.999     25 0.736 0.787 0.762 0.572     12.5 0.447 0.454 0.451 0.261     6.25 0.311 0.325 0.318 0.128     3.12 0.240 0.251 0.246 0.056     0 0.189 0.191 0.190 　         .

货期

3-5 working days