产品描述
CUSABIO's bFGF mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse bFGF in serum, plasma, cell culture supernates, or tissue homogenates. This assay uses an antibody specific for mouse bFGF coated on a 96-well plate. bFGF present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated bFGF antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of bFGF bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.
bFGF, also called FGF2, is a powerful angiogenic molecule that stimulates smooth muscle cell growth, wound healing, and tissue repair. In addition, FGF2 may stimulate hematopoiesis and may play an important role in the differentiation and/or function of the nervous system, eyes, and skeleton. Together with heparin or heparin sulfate proteoglycan, FGF2 activates FGFRs, inducing its pleiotropic effects in different tissues and organs, including potent angiogenic effects and important roles in the differentiation and function of the central nervous system (CNS). FGF2 plays a critical role in the development of the CNS. FGF2/FGFR1 signaling has been a hotspot of therapeutic development for neurodegenerative diseases such as multiple sclerosis, Alzheimer’s disease, and Parkinson’s disease.
别名
Fgf2 ELISA Kit; Fgf-2Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit
缩写
FGF2
Uniprot No
P15655
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 89-100 | |
| 1:2 | Average % | 94 | |
| Range % | 90-99 | |
| 1:4 | Average % | 97 | |
| Range % | 92-103 | |
| 1:8 | Average % | 94 | |
| Range % | 87-98 | |
回收率
| The recovery of mouse bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 87-95 | |
| EDTA plasma (n=4) | 96 | 92-101 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.895 | 2.863 | 2.879 | 2.711 | | | 100 | 2.455 | 2.364 | 2.410 | 2.242 | | | 50 | 1.995 | 1.976 | 1.986 | 1.818 | | | 25 | 1.556 | 1.532 | 1.544 | 1.376 | | | 12.5 | 1.011 | 0.968 | 0.990 | 0.822 | | | 6.25 | 0.452 | 0.427 | 0.440 | 0.272 | | | 3.12 | 0.292 | 0.284 | 0.288 | 0.120 | | | 0 | 0.169 | 0.167 | 0.168 | | |
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货期
3-5 working days
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