产品描述
CUSABIO’s Human Interferon α (IFN-α) ELISA Kit can be used to quantitatively measure the levels of IFN-α in various biological solutions, including serum, plasma, cell culture supernates, urine, and tissue homogenates. The assay uses the sandwich ELISA mechanism in combination with the chromogenic reaction to complete the detection. The solution color develops in proportion to the IFN-α contents in the sample, and the color intensity can be measured at 450 nm via a microplate reader. The kit is featured with high sensitivity, excellent specificity, the precision of less than 10%, high recovery, good linearity, and high consistency between batches.
The IFN-α belongs to the type I IFNs family and it is predominantly involved in innate immunity. IFN-α is secreted by immune (lymphocytes, NK cells, B-cells, T-cells, and macrophages) and non-immune cells (fibroblasts, endothelial cells, osteoblasts, and others) in response to the viral infection. IFN-α signals through the interaction with its receptor IFNAR, inducing the activation of the JAK and STAT signaling pathway and thus leading to the expression of related genes. These gene products exert anti-viral, anti-proliferative, anti-tumor, or immunomodulatory effects. In addition to its direct anti-viral effects, IFN-α can strongly regulate innate and adaptive immune responses in the host. It enhances the proliferation, cytotoxicity, and IFN-γ secretion of NK cells. It also up-regulates the expression of major histocompatibility complexes class I and II on antigen-presenting cells and promotes cross-presentation of viral antigens to CD8+ T-cells by DCs, which enhances adaptive immune responses.
别名
IFL ELISA Kit; IFN alpha ELISA Kit; IFN alpha 1/13 ELISA Kit; IFN alpha 1b ELISA Kit; IFN alphaD ELISA Kit; IFN ELISA Kit; IFN-alpha-1/13 ELISA Kit; IFNA@ ELISA Kit; IFNA1 ELISA Kit; IFNA1_HUMAN ELISA Kit; IFNA13 ELISA Kit; Interferon alpha 1 ELISA Kit; Interferon alpha-1/13 ELISA Kit; Interferon alpha-D ELISA Kit; Interferon alpha1 ELISA Kit; Interferon, alpha 13 ELISA Kit; LeIF D ELISA Kit
缩写
IFN-
Uniprot No
P01562
灵敏度
3.12 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 84-99 | |
| 1:2 | Average % | 90 | |
| Range % | 85-95 | |
| 1:4 | Average % | 100 | |
| Range % | 95-104 | |
| 1:8 | Average % | 91 | |
| Range % | 88-98 | |
回收率
| The recovery of human IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 85-99 | |
| EDTA plasma (n=4) | 93 | 85-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 800 | 1.875 | 1.952 | 1.914 | 1.777 | | | 400 | 1.484 | 1.464 | 1.474 | 1.337 | | | 200 | 1.062 | 1.046 | 1.054 | 0.917 | | | 100 | 0.768 | 0.742 | 0.755 | 0.618 | | | 50 | 0.473 | 0.492 | 0.483 | 0.346 | | | 25 | 0.345 | 0.361 | 0.353 | 0.216 | | | 12.5 | 0.256 | 0.272 | 0.264 | 0.127 | | | 0 | 0.136 | 0.138 | 0.137 | | |
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货期
3-5 working days
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