产品描述
This Human GP-BB ELISA Kit was designed for the quantitative measurement of Human GP-BB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
缩写
GP-BB
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human GP-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 91 | |
| Range % | 85-98 | |
| 1:2 | Average % | 96 | |
| Range % | 90-102 | |
| 1:4 | Average % | 95 | |
| Range % | 90-101 | |
| 1:8 | Average % | 95 | |
| Range % | 84-102 | |
回收率
| The recovery of human GP-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-100 | |
| EDTA plasma (n=4) | 96 | 92-105 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 40 | 3.047 | 3.108 | 3.078 | 2.901 | | | 20 | 2.475 | 2.557 | 2.516 | 2.339 | | | 10 | 1.839 | 1.785 | 1.812 | 1.635 | | | 5 | 1.128 | 1.124 | 1.126 | 0.949 | | | 2.5 | 0.724 | 0.719 | 0.722 | 0.545 | | | 1.25 | 0.496 | 0.468 | 0.482 | 0.305 | | | 0.625 | 0.330 | 0.332 | 0.331 | 0.154 | | | 0 | 0.177 | 0.176 | 0.177 | | |
|
货期
3-5 working days