产品描述
This Chicken IFNA1 ELISA Kit was designed for the quantitative measurement of Chicken IFNA1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .
别名
IFNA1; ELISA Kit; IFNA2 ELISA Kit; Interferon type A1/A2 ELISA Kit
缩写
IFN-α
Uniprot No
P42165
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 83 | |
| Range % | 80-95 | |
| 1:2 | Average % | 95 | |
| Range % | 91-101 | |
| 1:4 | Average % | 93 | |
| Range % | 86-98 | |
| 1:8 | Average % | 95 | |
| Range % | 82-98 | |
回收率
| The recovery of chicken IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 90-100 | |
| EDTA plasma (n=4) | 96 | 92-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 1.726 | 1.745 | 1.736 | 1.631 | | | 100 | 1.336 | 1.327 | 1.332 | 1.227 | | | 50 | 0.957 | 0.976 | 0.967 | 0.862 | | | 25 | 0.621 | 0.631 | 0.626 | 0.521 | | | 12.5 | 0.437 | 0.409 | 0.423 | 0.318 | | | 6.25 | 0.303 | 0.301 | 0.302 | 0.197 | | | 3.12 | 0.234 | 0.244 | 0.239 | 0.134 | | | 0 | 0.107 | 0.102 | 0.105 | | |
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货期
3-5 working days