产品描述
This Human HSPA1B ELISA Kit was designed for the quantitative measurement of Human HSPA1B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.
别名
HSPA1B ELISA Kit; HSP72 ELISA Kit; Heat shock 70 kDa protein 1B ELISA Kit; Heat shock 70 kDa protein 2 ELISA Kit; HSP70-2 ELISA Kit; HSP70.2 ELISA Kit
缩写
HSPA1B
Uniprot No
P0DMV9
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 85-100 | |
| 1:2 | Average % | 87 | |
| Range % | 82-93 | |
| 1:4 | Average % | 98 | |
| Range % | 95-100 | |
| 1:8 | Average % | 92 | |
| Range % | 88-94 | |
回收率
| The recovery of human HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 100 | 95-106 | |
| EDTA plasma (n=4) | 92 | 87-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.588 | 2.521 | 2.555 | 2.445 | | | 50 | 1.745 | 1.676 | 1.711 | 1.601 | | | 25 | 1.133 | 1.112 | 1.123 | 1.013 | | | 12.5 | 0.667 | 0.621 | 0.644 | 0.534 | | | 6.25 | 0.401 | 0.399 | 0.400 | 0.290 | | | 3.12 | 0.238 | 0.233 | 0.236 | 0.126 | | | 1.56 | 0.167 | 0.159 | 0.163 | 0.053 | | | 0 | 0.112 | 0.108 | 0.110 | | |
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货期
3-5 working days