产品描述
This rat gastrin (GAST) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat gastrin in multiple samples, including serum, plasma, cell culture supernates, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of gastrin bound in the initial step.
Gastrin is a peptide hormone that is produced in the G cells of the gastric antrum and duodenum and is then released into the blood circulation. Gastrin exerts its actions by binding to the CCK-B on the parietal and enterochromaffin cells of the gastric body. It is trophic for normal gastrointestinal epithelium. It primarily contributes to increasing gastric mucosal growth, gastric motility, and secretion of hydrochloric acid (HCl) into the stomach. Studies have shown that gastrin is involved in the stimulation of growth of several gastrointestinal cancers including gastric cancer.
别名
Gast ELISA Kit; Gas ELISA Kit; Gastrin [Cleaved into: Big gastrin ELISA Kit; Gastrin-34 ELISA Kit; G34); Gastrin] ELISA Kit
缩写
GAST
Uniprot No
P04563
灵敏度
0.078 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat gastrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 90 | |
| Range % | 85-95 | |
| 1:200 | Average % | 94 | |
| Range % | 90-98 | |
| 1:400 | Average % | 99 | |
| Range % | 95-104 | |
| 1:800 | Average % | 102 | |
| Range % | 98-107 | |
回收率
| The recovery of rat gastrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 88-96 | |
| EDTA plasma (n=4) | 100 | 95-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.496 | 2.537 | 2.517 | 2.390 | | | 10 | 2.181 | 2.192 | 2.187 | 2.060 | | | 5 | 1.795 | 1.741 | 1.768 | 1.641 | | | 2.5 | 1.353 | 1.313 | 1.333 | 1.206 | | | 1.25 | 0.878 | 0.898 | 0.888 | 0.761 | | | 0.625 | 0.369 | 0.366 | 0.368 | 0.241 | | | 0.312 | 0.242 | 0.259 | 0.251 | 0.124 | | | 0 | 0.123 | 0.130 | 0.127 | | |
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货期
3-5 working days
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