产品描述
This Chicken GPX-8 ELISA Kit was designed for the quantitative measurement of Chicken GPX-8 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 mU/mL-2000 mU/mL and the sensitivity is 7.8 mU/mL.
缩写
GPX-8
Uniprot No
R4GGB2
灵敏度
7.8 mU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken GPX-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
98 |
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| Range % |
94-103 |
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| 1:200 |
Average % |
86 |
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| Range % |
83-90 |
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| 1:400 |
Average % |
104 |
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| Range % |
100-108 |
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| 1:800 |
Average % |
89 |
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| Range % |
85-93 |
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回收率
| The recovery of chicken GPX-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
107 |
103-110 |
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| EDTA plasma (n=4) |
84 |
80-88 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| mU/ml |
OD1 |
OD2 |
Average |
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| 2000 |
0.111 |
0.115 |
0.113 |
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| 1000 |
0.208 |
0.210 |
0.209 |
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| 500 |
0.373 |
0.390 |
0.382 |
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| 250 |
0.642 |
0.638 |
0.640 |
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| 125 |
0.956 |
0.961 |
0.959 |
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| 62.5 |
1.146 |
1.200 |
1.173 |
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| 31.25 |
1.417 |
1.431 |
1.424 |
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| 0 |
1.634 |
1.645 |
1.640 |
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货期
3-5 working days