产品描述
This Mouse GDF2 ELISA Kit was designed for the quantitative measurement of Mouse GDF2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 pg/mL-20 pg/mL and the sensitivity is 0.078 pg/mL.
别名
Gdf2 ELISA Kit; Bmp9Growth/differentiation factor 2 ELISA Kit; GDF-2 ELISA Kit; Bone morphogenetic protein 9 ELISA Kit; BMP-9 ELISA Kit
缩写
GDF2
Uniprot No
Q9WV56
灵敏度
0.078 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 100 | |
| Range % | 91-104 | |
| 1:2 | Average % | 104 | |
| Range % | 97-108 | |
| 1:4 | Average % | 98 | |
| Range % | 89-102 | |
| 1:8 | Average % | 94 | |
| Range % | 89-99 | |
回收率
| The recovery of mouse BMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 99 | 89-103 | |
| EDTA plasma (n=4) | 94 | 85-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.266 | 2.231 | 2.249 | 2.081 | | | 10 | 1.389 | 1.296 | 1.343 | 1.175 | | | 5 | 0.850 | 0.834 | 0.842 | 0.674 | | | 2.5 | 0.582 | 0.531 | 0.557 | 0.389 | | | 1.25 | 0.422 | 0.441 | 0.432 | 0.264 | | | 0.625 | 0.316 | 0.303 | 0.310 | 0.142 | | | 0.312 | 0.249 | 0.233 | 0.241 | 0.073 | | | 0 | 0.170 | 0.166 | 0.168 | | |
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货期
3-5 working days