产品描述
The rat VEGFR-1 ELISA Kit is engineered for accurate measurement of rat VEGFR-1 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the VEGFR-1 content in the sample. The color intensity is positively correlated with VEGFR-1 content in the sample. The VEGFR-1 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.
VEGFR-1, also called Flt-1, is the exclusive VEGFR present on the surface of monocytes and mediates a chemotactic response to VEGF-A and tissue factor induction. In myeloid cells, VEGFR-1 mediates monocyte migration and has a regulatory role in the development of inflammatory diseases such as rheumatoid arthritis and arteriosclerosis. In vascular smooth muscle cells (SMCs), VEGFR-1 is inducibly expressed by vascular injury and participates in neointimal development. VEGFR-1 expression has frequently been detected in melanoma and is also found in other cancer types. Upon activation by VEGF-A or PlGF, VEGFR-1 promotes cell survival, migration, invasiveness, and chemoresistance of melanoma cells.
别名
Flt1 ELISA Kit; Flt-1 ELISA Kit; Vegfr1 ELISA Kit; Vascular endothelial growth factor receptor 1 ELISA Kit; VEGFR-1 ELISA Kit; EC 2.7.10.1 ELISA Kit; Fms-like tyrosine kinase 1 ELISA Kit; FLT-1 ELISA Kit; Tyrosine-protein kinase receptor FLT ELISA Kit
缩写
FLT1
Uniprot No
P53767
灵敏度
0.39 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGFR-1/Flt1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
92-101 |
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| 1:2 |
Average % |
103 |
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| Range % |
99-110 |
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| 1:4 |
Average % |
88 |
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| Range % |
84-92 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-100 |
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回收率
| The recovery of rat VEGFR-1/Flt1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
90-98 |
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| EDTA plasma (n=4) |
84 |
80-88 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.621 |
2.557 |
2.589 |
2.412 |
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| 50 |
2.005 |
1.946 |
1.976 |
1.799 |
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| 25 |
1.286 |
1.332 |
1.309 |
1.132 |
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| 12.5 |
0.893 |
0.859 |
0.876 |
0.699 |
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| 6.25 |
0.514 |
0.521 |
0.518 |
0.341 |
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| 3.12 |
0.397 |
0.408 |
0.403 |
0.226 |
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| 1.56 |
0.260 |
0.252 |
0.256 |
0.079 |
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| 0 |
0.179 |
0.174 |
0.177 |
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货期
3-5 working days
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