产品描述
This Dog IFNG ELISA Kit was designed for the quantitative measurement of Dog IFNG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
别名
IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写
IFNG
Uniprot No
P42161
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 90-104 | |
| 1:2 | Average % | 96 | |
| Range % | 92-102 | |
| 1:4 | Average % | 95 | |
| Range % | 89-102 | |
| 1:8 | Average % | 97 | |
| Range % | 90-101 | |
回收率
| The recovery of dog IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 93-100 | |
| EDTA plasma (n=4) | 96 | 89-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 1.885 | 1.853 | 1.869 | 1.766 | | | 100 | 1.226 | 1.308 | 1.267 | 1.164 | | | 50 | 0.891 | 0.924 | 0.908 | 0.805 | | | 25 | 0.636 | 0.686 | 0.661 | 0.558 | | | 12.5 | 0.407 | 0.425 | 0.416 | 0.313 | | | 6.25 | 0.310 | 0.318 | 0.314 | 0.211 | | | 3.12 | 0.248 | 0.252 | 0.250 | 0.147 | | | 0 | 0.102 | 0.104 | 0.103 | | |
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货期
3-5 working days