产品描述
This Human GH Ab ELISA Kit was designed for the quantitative measurement of Human GH Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 2 pg/mL.
缩写
GH Ab
灵敏度
2 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Developmental Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human anti-GH antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
82-105 |
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| 1:2 |
Average % |
94 |
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| Range % |
84-105 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-101 |
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| 1:8 |
Average % |
95 |
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| Range % |
82-105 |
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回收率
| The recovery of human anti-GH antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
82-105 |
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| EDTA plasma (n=4) |
93 |
84-102 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.604 |
2.573 |
2.589 |
2.448 |
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| 250 |
1.994 |
2.109 |
2.052 |
1.911 |
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| 125 |
1.382 |
1.361 |
1.372 |
1.231 |
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| 62.5 |
0.852 |
0.845 |
0.849 |
0.708 |
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| 31.2 |
0.524 |
0.516 |
0.520 |
0.379 |
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| 15.6 |
0.356 |
0.368 |
0.362 |
0.221 |
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| 7.8 |
0.242 |
0.253 |
0.248 |
0.107 |
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| 0 |
0.145 |
0.136 |
0.141 |
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货期
3-5 working days