产品描述
This Human IDS ELISA Kit was designed for the quantitative measurement of Human IDS protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.
别名
Alpha L iduronate sulfate sulfatase ELISA Kit; Alpha-L-iduronate sulfate sulfatase ELISA Kit; AW214631 ELISA Kit; Ids ELISA Kit; IDS_HUMAN ELISA Kit; Iduronate 2 sulfatase 14 kDa chain ELISA Kit; Iduronate 2 sulfatase 42 kDa chain ELISA Kit; Iduronate 2 sulfatase ELISA Kit; Iduronate 2-sulfatase 14 kDa chain ELISA Kit; Iduronate sulfatase ELISA Kit; Idursulfase ELISA Kit; MPS2 ELISA Kit; RP23-29M4.1 ELISA Kit; SIDS ELISA Kit
缩写
IDS
Uniprot No
P22304
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Tags & Cell Markers
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IDS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 87-95 | |
| 1:2 | Average % | 89 | |
| Range % | 85-94 | |
| 1:4 | Average % | 95 | |
| Range % | 89-99 | |
| 1:8 | Average % | 102 | |
| Range % | 98-105 | |
回收率
| The recovery of human IDS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 93-102 | |
| EDTA plasma (n=4) | 87 | 80-92 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.257 | 2.357 | 2.307 | 2.201 | | | 1000 | 1.976 | 1.872 | 1.924 | 1.818 | | | 500 | 1.532 | 1.578 | 1.555 | 1.449 | | | 250 | 1.172 | 1.151 | 1.162 | 1.056 | | | 125 | 0.711 | 0.728 | 0.720 | 0.614 | | | 62.5 | 0.426 | 0.434 | 0.430 | 0.324 | | | 31.25 | 0.262 | 0.270 | 0.266 | 0.160 | | | 0 | 0.105 | 0.106 | 0.106 | | |
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货期
3-5 working days