产品描述
This Mouse CYP2E1 ELISA Kit was designed for the quantitative measurement of Mouse CYP2E1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.75 pg/mL.
别名
Cyp2e1 ELISA kit; Cyp2e ELISA kit; Cyp2e-1Cytochrome P450 2E1 ELISA kit; EC 1.14.14.1 ELISA kit; 4-nitrophenol 2-hydroxylase ELISA kit; EC 1.14.13.n7 ELISA kit; CYPIIE1 ELISA kit; Cytochrome P450-ALC ELISA kit; Cytochrome P450-J ELISA kit
缩写
CYP2E1
Uniprot No
Q05421
灵敏度
9.75 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CYP2E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 84 | | |
| Range % | 80-92 | | |
| 1:2 | Average % | 96 | | |
| Range % | 92-100 | | |
| 1:4 | Average % | 91 | | |
| Range % | 85-95 | | |
| 1:8 | Average % | 104 | | |
| Range % | 100-113 | | |
回收率
| The recovery of mouse CYP2E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 95 | 89-100 | | |
| EDTA plasma (n=4) | 92 | 88-96 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 2500 | 2.482 | 2.658 | 2.570 | 2.384 | | | | 1250 | 1.968 | 2.076 | 2.022 | 1.836 | | | | 625 | 1.418 | 1.503 | 1.461 | 1.275 | | | | 312 | 0.833 | 0.857 | 0.845 | 0.659 | | | | 156 | 0.555 | 0.521 | 0.538 | 0.352 | | | | 78 | 0.315 | 0.321 | 0.318 | 0.132 | | | | 39 | 0.235 | 0.244 | 0.240 | 0.054 | | | | 0 | 0.183 | 0.188 | 0.186 | | | |
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货期
3-5 working days