产品描述

This human IDO1 ELISA Kit is suitable for qualitatively determining human concentrations in multiple biological fluids, including human serum, plasma, and tissue homogenates in vitro. IDO1 a cytosolic enzyme with a heme (Fe2+) prosthetic group that catalyzes catalyze the oxidative catabolism of the least-abundant essential amino acid, L-Trp (L-tryptophan), along the kynurenine pathway. IDO1 converts the essential amino acid Trp to kynurenine (Kyn) by cleaving the 2,3-double bond of the indole ring while molecular oxygen merges into the unsealed molecule. It has been identified as a prominent immune regulatory enzyme that can regulate immune cell activation status and phenotype through enzyme-dependent deprivation of L-Trp and its conversion into the aryl hydrocarbon receptor ligand kynurenine or IDO1-mediated signaling. IDO1 also has pro-cancer effects. Overexpression of IDO1 has been detected in more than half of tumors using IDO1-related immunosuppressive mechanisms to promote their metastasis and survival.

This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human IDO1 in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-human IDO1 antibody. Biotin-labeled IDO1 antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the IDO1 in the human samples. IDO1 binds to the biotinylated anti-IDO1 human monoclonal antibody. And the biotin on the biotinylated anti-IDO1 human monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of IDO1 in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

别名

缩写

Uniprot No

灵敏度

反应时间

样本体积

检测波长

研究领域

测定原理

精密度

线性度

回收率

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 50 2.574 2.552 2.563 2.461 25 2.078 1.949 2.014 1.912 12.5 1.228 1.278 1.253 1.151 6.25 0.537 0.560 0.549 0.447 3.12 0.326 0.340 0.333 0.231 1.56 0.204 0.197 0.201 0.099 0.78 0.159 0.152 0.156 0.054 0 0.103 0.100 0.102

货期