产品描述

This Human HEXA ELISA Kit was designed for the quantitative measurement of Human HEXA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

Beta hexosaminidase alpha chain precursor ELISA Kit; Beta hexosaminidase subunit alpha ELISA Kit; Beta N acetylhexosaminidase ELISA Kit; Beta N acetylhexosaminidase subunit alpha ELISA Kit; Beta-hexosaminidase A ELISA Kit; Beta-hexosaminidase subunit alpha ELISA Kit; Beta-N-acetylhexosaminidase subunit alpha ELISA Kit; Hexa ELISA Kit; HEXA_HUMAN ELISA Kit; Hexosaminidase A (alpha polypeptide) ELISA Kit; Hexosaminidase A alpha polypeptide ELISA Kit; Hexosaminidase A ELISA Kit; Hexosaminidase subunit A ELISA Kit; MGC99608 ELISA Kit; N acetyl beta glucosaminidase ELISA Kit; N acetyl beta glucosaminidase subunit alpha ELISA Kit; N-acetyl-beta-glucosaminidase subunit alpha ELISA Kit; TSD ELISA Kit

缩写

HEXA

Uniprot No

P06865

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human HEXA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		101
		Range %		94-106
1:2		Average %		97
		Range %		91-103
1:4		Average %		93
		Range %		87-99
1:8		Average %		88
		Range %		85-92

回收率

The recovery of human HEXA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		93-102
EDTA plasma (n=4)		87		80-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected     1000 2.245 2.164 2.205 2.053     500 1.407 1.295 1.351 1.199     250 0.832 0.812 0.822 0.670     125 0.485 0.499 0.492 0.340     62.5 0.334 0.316 0.325 0.173     31.2 0.231 0.226 0.229 0.077     15.6 0.192 0.199 0.196 0.044     0 0.152 0.151 0.152

货期

3-5 working days