产品描述
FT4(游离甲状腺素):FT4是甲状腺素中的游离形式,是人体代谢功能的重要调节因子之一。它是由甲状腺腺体分泌的主要甲状腺激素之一,与T3一样,对细胞的能量代谢、生长发育、心血管、神经等系统的正常功能都有着重要的影响。FT4的含量水平是甲状腺功能的重要指标,甲状腺功能亢进时FT4水平会升高,甲状腺功能减退时FT4水平则会降低。
华美生物所提供的Pig free thyroxine (FT4) ELISA kit属于ELISA检测试剂盒,采用竞争法定量检测猪 血清、血浆、细胞培养上清、组织匀浆样本中的FT4,其灵敏度为1.08 pmol/L,检测范围为4 pmol/L-64 pmol/L。
缩写
FT4
灵敏度
1.08 pmol/L
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
85-99 |
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| 1:2 |
Average % |
95 |
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| Range % |
87-106 |
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| 1:4 |
Average % |
96 |
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| Range % |
90-110 |
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| 1:8 |
Average % |
91 |
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| Range % |
86-98 |
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回收率
| The recovery of pig FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
93-103 |
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| EDTA plasma (n=4) |
96 |
93-102 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pmol/L |
OD1 |
OD2 |
Average |
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| 4 |
2.771 |
2.736 |
2.754 |
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| 8 |
1.459 |
1.442 |
1.451 |
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| 16 |
0.827 |
0.865 |
0.846 |
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| 32 |
0.575 |
0.565 |
0.570 |
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| 64 |
0.318 |
0.307 |
0.313 |
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货期
3-5 working days