产品描述
This Mouse VEGFR-1/Flt1 ELISA Kit was designed for the quantitative measurement of Mouse VEGFR-1/Flt1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.
别名
Flt1 ELISA Kit; Emrk2 ELISA Kit; Flt ELISA Kit; Vegfr1 ELISA Kit; Vascular endothelial growth factor receptor 1 ELISA Kit; VEGFR-1 ELISA Kit; EC 2.7.10.1 ELISA Kit; Embryonic receptor kinase 2 ELISA Kit; Fms-like tyrosine kinase 1 ELISA Kit; FLT-1 ELISA Kit; Tyrosine-protein kinase receptor FLT ELISA Kit
缩写
FLT1
Uniprot No
P35969
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGFR-1/Flt1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-95 |
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| 1:2 |
Average % |
103 |
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| Range % |
100-106 |
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| 1:4 |
Average % |
85 |
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| Range % |
80-90 |
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| 1:8 |
Average % |
104 |
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| Range % |
100-107 |
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回收率
| The recovery of mouse VEGFR-1/Flt1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
84 |
80-90 |
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| EDTA plasma (n=4) |
108 |
104-112 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.602 |
2.631 |
2.617 |
2.495 |
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| 50 |
2.003 |
2.068 |
2.036 |
1.914 |
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| 25 |
1.339 |
1.361 |
1.350 |
1.228 |
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| 12.5 |
0.785 |
0.779 |
0.782 |
0.660 |
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| 6.25 |
0.452 |
0.461 |
0.457 |
0.335 |
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| 3.12 |
0.284 |
0.291 |
0.288 |
0.166 |
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| 1.56 |
0.186 |
0.187 |
0.187 |
0.065 |
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| 0 |
0.120 |
0.123 |
0.122 |
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货期
3-5 working days