产品描述
This Rat CXCL10 ELISA Kit was designed for the quantitative measurement of Rat CXCL10 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 781.25 pg/mL-50000 pg/mL and the sensitivity is 195 pg/mL .
别名
Cxcl10 ELISA kit; Inp10 ELISA kit; Mob1 ELISA kit; Scyb10C-X-C motif chemokine 10 ELISA kit; 10 kDa interferon gamma-induced protein ELISA kit; Gamma-IP10 ELISA kit; IP-10 ELISA kit; Interferon-inducible protein 10 ELISA kit; Protein Mob-1 ELISA kit; Small-inducible cytokine B10 ELISA kit
缩写
CXCL10
Uniprot No
P48973
灵敏度
195 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 84-92 | |
| 1:2 | Average % | 94 | |
| Range % | 90-98 | |
| 1:4 | Average % | 99 | |
| Range % | 95-104 | |
| 1:8 | Average % | 102 | |
| Range % | 96-108 | |
回收率
| The recovery of rat IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 93-96 | |
| EDTA plasma (n=4) | 100 | 95-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 50000 | 2.556 | 2.581 | 2.569 | 2.437 | | | 25000 | 1.748 | 1.769 | 1.759 | 1.627 | | | 12500 | 1.002 | 1.085 | 1.044 | 0.912 | | | 6250 | 0.663 | 0.699 | 0.681 | 0.549 | | | 3125 | 0.396 | 0.387 | 0.392 | 0.260 | | | 1562.5 | 0.284 | 0.271 | 0.278 | 0.146 | | | 781.25 | 0.219 | 0.230 | 0.225 | 0.093 | | | 0 | 0.131 | 0.133 | 0.132 | | |
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货期
3-5 working days