产品描述

The product CSB-EL007680HU is a sandwich ELISA kit developed to measure concentrations of human ENPP2 in serum, plasma, or tissue homogenates. This assay uses the competitive technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops negatively to the amount of ENPP2 in samples. The color intensity is measured at 450 nm via a microplate reader.

ENPP2, also known as autotaxin (ATX), is an enzyme present in blood circulation that is responsible for the conversion of lysophosphatidylcholine (LPC) to lysophosphatidic acid (LPA). LPA is a phospholipid mediator that elicits growth-factor-like responses in almost all cell types, including cell growth, survival, differentiation, and motility. ENPP2/LPA promotes several physiological and pathological processes, including in pulmonary fibrosis, thus serving as attractive drug targets. Increased ENPP2 expression has been detected in various types of cancer, including mammary, thyroid, hepatocellular, and renal cell carcinomas, non-small-cell lung cancer, as well as glioblastoma and neuroblastoma.

别名

ATX ELISA Kit; ATX X ELISA Kit; Autotaxin ELISA Kit; Autotaxin t ELISA Kit; E NPP 2 ELISA Kit; E-NPP 2 ELISA Kit; Ectonucleotide pyrophosphatase/phosphodiesterase 2 ELISA Kit; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 ELISA Kit; Enpp2 ELISA Kit; ENPP2_HUMAN ELISA Kit; Extracellular lysophospholipase D ELISA Kit; FLJ26803 ELISA Kit; LysoPLD ELISA Kit; NPP2 ELISA Kit; PD IALPHA ELISA Kit; PDNP2 ELISA Kit; Phosphodiesterase I alpha ELISA Kit; Phosphodiesterase I/nucleotide pyrophosphatase 2 ELISA Kit; Plasma lysophospholipase D ELISA Kit

缩写

ENPP2

Uniprot No

Q13822

灵敏度

31.25 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ENPP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		83-103
1:2		Average %		96
		Range %		89-103
1:4		Average %		90
		Range %		86-100
1:8		Average %		89
		Range %		82-101

回收率

The recovery of human ENPP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		82-104
EDTA plasma (n=4)		97		85-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average     2000 0.145 0.142 0.144     750 0.270 0.283 0.277     375 0.432 0.410 0.421     125 0.571 0.592 0.582     25 1.037 1.007 1.022     0 2.432 2.411 2.422

货期

3-5 working days