产品描述
This Rabbit EDN1 ELISA Kit was designed for the quantitative measurement of Rabbit EDN1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.
别名
EDN1Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit
缩写
EDN1
Uniprot No
P29560
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 80-92 | |
| 1:2 | Average % | 100 | |
| Range % | 91-105 | |
| 1:4 | Average % | 96 | |
| Range % | 92-104 | |
| 1:8 | Average % | 94 | |
| Range % | 86-98 | |
回收率
| The recovery of rabbit ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-99 | |
| EDTA plasma (n=4) | 98 | 90-106 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.630 | 2.456 | 2.543 | 2.458 | | | 200 | 2.111 | 2.076 | 2.094 | 2.009 | | | 100 | 1.459 | 1.443 | 1.451 | 1.366 | | | 50 | 0.816 | 0.769 | 0.793 | 0.708 | | | 25 | 0.415 | 0.389 | 0.402 | 0.317 | | | 12.5 | 0.218 | 0.203 | 0.211 | 0.126 | | | 6.25 | 0.147 | 0.149 | 0.148 | 0.063 | | | 0 | 0.088 | 0.082 | 0.085 | | |
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货期
3-5 working days