产品描述
This Human HTT ELISA Kit was designed for the quantitative measurement of Human HTT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.125 pg/mL.
别名
AI256365 ELISA Kit; C430023I11Rik ELISA Kit; HD ELISA Kit; HD protein ELISA Kit; HD_HUMAN ELISA Kit; HDH ELISA Kit; HTT ELISA Kit; Huntingtin ELISA Kit; HUNTINGTON CHOREA ELISA Kit; Huntington disease protein ELISA Kit; Huntington's disease protein homolog ELISA Kit; IT 15 ELISA Kit; IT15 ELISA Kit; OTTMUSP00000026909 ELISA Kit; ZHD ELISA Kit
缩写
HTT
Uniprot No
P42858
灵敏度
3.125 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human HTT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 87 | | |
| Range % | 82-91 | | |
| 1:2 | Average % | 94 | | |
| Range % | 89-98 | | |
| 1:4 | Average % | 107 | | |
| Range % | 103-110 | | |
| 1:8 | Average % | 110 | | |
| Range % | 104-114 | | |
回收率
| The recovery of human HTT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 95 | 91-99 | | |
| EDTA plasma (n=4) | 103 | 98-108 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 800 | 2.245 | 2.198 | 2.222 | 2.134 | | | | 400 | 1.742 | 1.765 | 1.754 | 1.666 | | | | 200 | 1.278 | 1.301 | 1.290 | 1.202 | | | | 100 | 0.890 | 0.891 | 0.891 | 0.803 | | | | 50 | 0.615 | 0.604 | 0.610 | 0.522 | | | | 25 | 0.411 | 0.421 | 0.416 | 0.328 | | | | 12.5 | 0.283 | 0.286 | 0.285 | 0.197 | | | | 0 | 0.089 | 0.086 | 0.088 | | | |
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货期
3-5 working days