产品描述

The product CSB-EL007399HU is a sandwich ELISA kit developed to measure concentrations of human EDIL3 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of EDIL3 in samples. The color intensity is measured at 450 nm via a microplate reader.

As a pro-angiogenic factor, EDIL3 mediates the immune and anti-inflammatory response. EDIL3 acts as a regulator of endothelial cell adhesion and migration. Additionally, Edil3 has been described as an autocrine or paracrine secreted protein that participates in endothelial cell migration, angiogenesis, and branching morphogenesis of blood vessels. It has been reported that EDIL3 is overexpressed in several tumor types, including bladder, pancreas, breast, and liver carcinomas. Overexpression of EDIL3 promotes carcinogenesis by reducing apoptosis in cancer cells and stimulating cancer vascularization. It is associated with tumor progression and poor prognosis.

别名

DEL1 ELISA Kit; Developmental endothelial locus 1 ELISA Kit; Developmentally regulated endothelial cell locus 1 protein ELISA Kit; Developmentally-regulated endothelial cell locus 1 protein ELISA Kit; Edil3 ELISA Kit; EDIL3_HUMAN ELISA Kit; EGF like repeat and discoidin I like domain containing protein 3 ELISA Kit; EGF like repeats and discoidin I like domains 3 ELISA Kit; EGF like repeats and discoidin I like domains protein 3 ELISA Kit; EGF-like repeat and discoidin I-like domain-containing protein 3 ELISA Kit; Integrin binding protein DEL1 ELISA Kit; Integrin-binding protein DEL1 ELISA Kit

缩写

EDIL3

Uniprot No

O43854

灵敏度

31.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human EDIL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		87
		Range %		80-95
1:2		Average %		95
		Range %		89-100
1:4		Average %		99
		Range %		91-110
1:8		Average %		94
		Range %		88-98

回收率

The recovery of human EDIL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		80-98
EDTA plasma (n=4)		96		92-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 8000 2.033 1.872 1.953 1.765 4000 1.598 1.476 1.537 1.349 2000 1.077 1.014 1.046 0.858 1000 0.774 0.729 0.752 0.564 500 0.513 0.494 0.504 0.316 250 0.374 0.359 0.367 0.179 125 0.291 0.278 0.285 0.097 0 0.190 0.186 0.188

货期

3-5 working days