产品描述

This Human GAPDH/G3PDH ELISA Kit was designed for the quantitative measurement of Human GAPDH/G3PDH protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

别名

38 kDa BFA-dependent ADP-ribosylation substrate ELISA Kit; aging associated gene 9 protein ELISA Kit; Aging-associated gene 9 protein ELISA Kit; BARS-38 ELISA Kit; cb609 ELISA Kit; EC 1.2.1.12 ELISA Kit; Epididymis secretory sperm binding protein Li 162eP ELISA Kit; G3P_HUMAN ELISA Kit; G3PD ELISA Kit; G3PDH ELISA Kit; GAPD ELISA Kit; GAPDH ELISA Kit; Glyceraldehyde 3 phosphate dehydrogenase ELISA Kit; glyceraldehyde 3-PDH ELISA Kit; Glyceraldehyde-3-phosphate dehydrogenase ELISA Kit; HEL-S-162eP ELISA Kit; KNC-NDS6 ELISA Kit; MGC102544 ELISA Kit; MGC102546 ELISA Kit; MGC103190 ELISA Kit; MGC103191 ELISA Kit; MGC105239 ELISA Kit; MGC127711 ELISA Kit; MGC88685 ELISA Kit; OCAS; p38 component ELISA Kit; OCT1 coactivator in S phase; 38-KD component ELISA Kit; peptidyl cysteine S nitrosylase GAPDH ELISA Kit; Peptidyl-cysteine S-nitrosylase GAPDH ELISA Kit; wu:fb33a10 ELISA Kit

缩写

GAPDH/G3PDH

Uniprot No

P04406

灵敏度

7.81 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human GAPDH/G3PDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

88

Range %

81-92

1:2

Average %

98

Range %

94-101

1:4

Average %

99

Range %

94-103

1:8

Average %

95

Range %

89-98

回收率

The recovery of human GAPDH/G3PDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type			Average % Recovery				Range
Serum (n=5)			93				89-96
EDTA plasma (n=4)			96				92-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.488 2.321 2.405 2.264 1000 1.899 1.776 1.838 1.697 500 1.234 1.274 1.254 1.113 250 0.700 0.688 0.694 0.553 125 0.464 0.451 0.458 0.317 62.5 0.238 0.242 0.240 0.099 31.25 0.197 0.192 0.195 0.054 0 0.138 0.144 0.141

货期

3-5 working days