产品描述
CTX- (C端肽交联端粒化Ⅰ型胶原) 是Ⅰ型胶原的降解产物,在体内反映骨代谢的情况,被广泛用于骨质疏松的诊断和疗效评估。CTX- 作为骨吸收的标志物,在骨疾病、肿瘤骨病、炎症性肠病等疾病的诊断和治疗过程中得到广泛应用。
华美生物所提供的Rat C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测大鼠血清、血浆、组织匀浆样本中的CTX-,其灵敏度为31.25 pg/mL,检测范围为25 pg/mL-2000 pg/mL。
别名
C-telopeptide of type 1 collagen,CTX-1,_x000D_C-telopeptide of type I collagen,CTX-I
缩写
CTX-
灵敏度
31.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:2 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:4 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:8 |
Average % |
84 |
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| Range % |
80-88 |
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回收率
| The recovery of rat CTX-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
92-102 |
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| EDTA plasma (n=4) |
86 |
82-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 2000 |
0.353 |
0.354 |
0.354 |
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| 750 |
0.468 |
0.475 |
0.472 |
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| 375 |
0.616 |
0.605 |
0.611 |
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| 125 |
1.011 |
0.995 |
1.003 |
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| 25 |
1.512 |
1.594 |
1.553 |
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| 0 |
2.013 |
2.114 |
2.064 |
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货期
3-5 working days