产品描述

This Human ELAVL1 ELISA Kit was designed for the quantitative measurement of Human ELAVL1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

别名

ELAV (embryonic lethal abnormal vision Drosophila) like 1 ELISA Kit; ELAV (embryonic lethal; abnormal vision; Drosophila) like 1 (Hu antigen R) ELISA Kit; ELAV like 1 ELISA Kit; ELAV like RNA binding protein 1 ELISA Kit; ELAV-like protein 1 ELISA Kit; ELAV1 ELISA Kit; ELAV1_HUMAN ELISA Kit; Elavl1 ELISA Kit; Embryonic lethal abnormal vision drosophila homolog like 1 ELISA Kit; Hu Antigen R ELISA Kit; Hu-antigen R ELISA Kit; Hua ELISA Kit; HuR ELISA Kit; MelG ELISA Kit

缩写

ELAVL1

Uniprot No

Q15717

灵敏度

6.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ELAVL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		89-99
1:2		Average %		96
		Range %		92-101
1:4		Average %		94
		Range %		88-98
1:8		Average %		92
		Range %		88-96

回收率

The recovery of human ELAVL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		94-102
EDTA plasma (n=4)		87		83-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1600 2.167 2.058 2.113 1.956 800 1.643 1.553 1.598 1.441 400 1.166 1.137 1.152 0.995 200 0.787 0.759 0.773 0.616 100 0.542 0.536 0.539 0.382 50 0.368 0.352 0.360 0.203 25 0.279 0.271 0.275 0.118 0 0.158 0.156 0.157

货期

3-5 working days