产品描述
This Bovine Vitamin D-binding protein (GC) ELISA Kit was designed for the quantitative measurement of Bovine Vitamin D-binding protein (GC) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.156 μg/mL-10 μg/mL and the sensitivity is 0.039 μg/mL.
别名
GCVitamin D-binding protein ELISA kit; DBP ELISA kit; VDB ELISA kit; Gc-globulin ELISA kit; Group-specific component ELISA kit
缩写
GC
Uniprot No
Q3MHN5
灵敏度
0.039 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
86 |
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| Range % |
82-92 |
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| 1:200 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:400 |
Average % |
92 |
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| Range % |
84-96 |
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| 1:800 |
Average % |
95 |
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| Range % |
90-100 |
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回收率
| The recovery of bovine GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
85-96 |
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| EDTA plasma (n=4) |
103 |
100-106 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/ml |
OD1 |
OD2 |
Average |
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| 10 |
0.103 |
0.104 |
0.104 |
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| 5 |
0.151 |
0.155 |
0.153 |
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| 2.5 |
0.275 |
0.292 |
0.284 |
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| 1.25 |
0.537 |
0.540 |
0.539 |
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| 0.625 |
0.981 |
0.923 |
0.952 |
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| 0.312 |
1.246 |
1.328 |
1.287 |
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| 0.156 |
1.588 |
1.724 |
1.656 |
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| 0 |
2.424 |
2.613 |
2.519 |
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货期
3-5 working days