产品描述
叶酸化学名为N-(4-羧基-2-氧代-1,2,3,4-四氢喹啉-6-基)-L-谷氨酸,是一种水溶性维生素。它在体内参与DNA和RNA的合成、维持神经系统的正常功能以及红细胞的形成。缺乏叶酸会导致巨幼红细胞贫血和神经系统疾病等健康问题。
华美生物所提供的Human Folic acid(FA) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测人血清、血浆样本中的FA,其灵敏度为6.25 pg/mL,检测范围为12.5 pg/mL-200 pg/mL。
别名
N/A
缩写
FA
灵敏度
6.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
89 |
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| Range % |
84-93 |
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| 1:40 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:80 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:160 |
Average % |
84 |
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| Range % |
80-88 |
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回收率
| The recovery of human FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
94-105 |
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| EDTA plasma (n=4) |
95 |
90-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 200 |
0.093 |
0.090 |
0.092 |
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| 100 |
0.227 |
0.208 |
0.218 |
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| 50 |
0.378 |
0.367 |
0.373 |
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| 25 |
0.618 |
0.637 |
0.628 |
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| 12.5 |
0.986 |
1.003 |
0.995 |
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| 0 |
1.485 |
1.429 |
1.457 |
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货期
3-5 working days