产品描述
GPX1 (Glutathione Peroxidase 1)是一种重要的抗氧化酶,能够催化还原型谷胱甘肽(GSH)对过氧化氢和其他过氧化物进行还原,从而保护细胞免受氧化损伤。GPX1的缺乏或活性下降与多种疾病的发生和发展密切相关,如心血管疾病、炎症性肠病等。因此,测定GPX1的含量可以用来评估机体抗氧化能力的水平,以及某些疾病的风险。
华美生物所提供的Rat Glutathione Peroxidase (GSH-PX) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆样本中的GPX1,其灵敏度为3.12 mIU/mL,检测范围为12.5 mIU/mL-800 mIU/mL。
别名
Gpx1Glutathione peroxidase 1 ELISA Kit; GPx-1 ELISA Kit; GSHPx-1 ELISA Kit; EC 1.11.1.9 ELISA Kit; Cellular glutathione peroxidase ELISA Kit
缩写
GPX1
Uniprot No
P04041
灵敏度
3.12 mIU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
92 |
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| Range % |
85-100 |
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| 1:400 |
Average % |
97 |
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| Range % |
94-101 |
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| 1:800 |
Average % |
95 |
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| Range % |
90-99 |
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| 1:1600 |
Average % |
93 |
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| Range % |
87-98 |
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回收率
| The recovery of rat GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
86-100 |
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| EDTA plasma (n=4) |
100 |
95-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mIU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.251 |
2.299 |
2.275 |
2.119 |
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| 400 |
1.845 |
1.834 |
1.840 |
1.684 |
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| 200 |
1.301 |
1.324 |
1.313 |
1.157 |
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| 100 |
0.892 |
0.876 |
0.884 |
0.728 |
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| 50 |
0.553 |
0.590 |
0.572 |
0.416 |
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| 25 |
0.395 |
0.381 |
0.388 |
0.232 |
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| 12.5 |
0.251 |
0.234 |
0.243 |
0.087 |
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| 0 |
0.155 |
0.157 |
0.156 |
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货期
3-5 working days