产品描述
This Human HLA-B27 ELISA Kit was designed for the quantitative measurement of Human HLA-B27 protein in serum, urine, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
缩写
HLA-B27
Uniprot No
P03989
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-B27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
80-98 |
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| 1:2 |
Average % |
86 |
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| Range % |
80-96 |
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| 1:4 |
Average % |
96 |
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| Range % |
92-100 |
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| 1:8 |
Average % |
100 |
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| Range % |
96-110 |
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回收率
| The recovery of human HLA-B27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.730 |
2.612 |
2.671 |
2.563 |
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| 10 |
2.361 |
2.241 |
2.301 |
2.193 |
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| 5 |
1.702 |
1.638 |
1.670 |
1.562 |
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| 2.5 |
0.933 |
0.871 |
0.902 |
0.794 |
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| 1.25 |
0.534 |
0.522 |
0.528 |
0.420 |
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| 0.625 |
0.324 |
0.318 |
0.321 |
0.213 |
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| 0.312 |
0.243 |
0.236 |
0.240 |
0.132 |
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| 0 |
0.109 |
0.107 |
0.108 |
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货期
3-5 working days