产品描述
The product CSB-E04723m is a sandwich ELISA kit developed to measure concentrations of mouse CXCL12 in serum, plasma, cell culture supernates, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of CXCL12 in samples. The color intensity is measured at 450 nm via a microplate reader.
CXCL12, also called SDF-1, is a homeostatic CXC chemokine that regulates hematopoietic cell trafficking and secondary lymphoid tissue architecture. CXCL12 exerts its various effects through its receptors, CXCR4 and CXCR7. The CXCL12/CXCR4/CXCR7 axis plays a pivotal role in tumor development, survival, angiogenesis, metastasis, and tumor microenvironment. CXCL12 is known to contribute a lot to renal development, especially in renal vascular formation. It is involved in various kidney diseases, such as acute kidney injury (AKI), diabetic kidney disease (DKD), lupus nephritis, kidney transplantation, and renal cell carcinoma (RCC).
别名
Cxcl12 ELISA Kit; Sdf1 ELISA Kit; Stromal cell-derived factor 1 ELISA Kit; SDF-1 ELISA Kit; 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 ELISA Kit; TPAR1 ELISA Kit; C-X-C motif chemokine 12 ELISA Kit; Pre-B cell growth-stimulating factor ELISA Kit; PBSF ELISA Kit; Thymic lymphoma cell-stimulating factor ELISA Kit; TLSF ELISA Kit
缩写
CXCL12
Uniprot No
P40224
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SDF-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 92-100 | |
| 1:2 | Average % | 102 | |
| Range % | 99-105 | |
| 1:4 | Average % | 89 | |
| Range % | 87-91 | |
| 1:8 | Average % | 94 | |
| Range % | 90-98 | |
回收率
| The recovery of mouse SDF-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 103 | 99-107 | |
| EDTA plasma (n=4) | 95 | 90-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.012 | 2.122 | 2.067 | 1.910 | | | 1000 | 1.368 | 1.356 | 1.362 | 1.205 | | | 500 | 0.812 | 0.841 | 0.827 | 0.670 | | | 250 | 0.455 | 0.432 | 0.444 | 0.287 | | | 125 | 0.301 | 0.302 | 0.302 | 0.145 | | | 62.5 | 0.258 | 0.264 | 0.261 | 0.104 | | | 31.25 | 0.201 | 0.203 | 0.202 | 0.045 | | | 0 | 0.156 | 0.158 | 0.157 | | |
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货期
3-5 working days
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