产品描述
This Mouse CYCS ELISA Kit was designed for the quantitative measurement of Mouse CYCS protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 34.3 pg/mL-2200 pg/mL and the sensitivity is 8.5 pg/mL.
别名
Cycs ELISA Kit; Cytochrome c ELISA Kit; somatic ELISA Kit
缩写
CYCS
Uniprot No
P62897
灵敏度
8.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Cyt-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 92 | | |
| Range % | 88-97 | | |
| 1:2 | Average % | 84 | | |
| Range % | 80-88 | | |
| 1:4 | Average % | 90 | | |
| Range % | 85-94 | | |
| 1:8 | Average % | 97 | | |
| Range % | 92-101 | | |
回收率
| The recovery of mouse Cyt-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 101 | 95-105 | | |
| EDTA plasma (n=4) | 87 | 83-91 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 2200 | 2.352 | 2.512 | 2.432 | 2.240 | | | | 1100 | 1.615 | 1.578 | 1.597 | 1.405 | | | | 550 | 1.073 | 1.023 | 1.048 | 0.856 | | | | 275 | 0.639 | 0.681 | 0.660 | 0.468 | | | | 137.5 | 0.461 | 0.489 | 0.475 | 0.283 | | | | 68.75 | 0.387 | 0.374 | 0.381 | 0.189 | | | | 34.3 | 0.318 | 0.305 | 0.312 | 0.120 | | | | 0 | 0.194 | 0.189 | 0.192 | | | |
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货期
3-5 working days