产品描述
高敏C反应蛋白(high-sensitivity C-reactive protein,hs-CRP),是一种由肝脏合成的血浆蛋白,主要是响应机体内的炎症和损伤。hs-CRP的含量水平通常很低,但在炎症、感染、心血管疾病等情况下,其含量会显著升高。因此,hs-CRP测定可以用于评估心血管疾病风险、监测炎症进程等。
华美生物所提供的Human high sensitivity C-Reactive Protein,hs-CRP ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、细胞培养上清、组织匀浆样本中的hs-CRP,其灵敏度为0.156 ng/mL,检测范围为0.625 ng/mL-40 ng/mL。
别名
Pentraxin 1, short ELISA Kit; C reactive protein ELISA Kit; C reactive protein pentraxin related ELISA Kit; C-reactive protein(1-205) ELISA Kit; CRP ELISA Kit; CRP_HUMAN ELISA Kit; MGC88244 ELISA Kit; Pentraxin 1 ELISA Kit; PTX 1 ELISA Kit; PTX1 ELISA Kit
缩写
hs-CRP
Uniprot No
P02741
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:400 |
Average % |
104 |
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| Range % |
100-108 |
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| 1:800 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:1600 |
Average % |
99 |
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| Range % |
93-104 |
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回收率
| The recovery of human CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-96 |
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| EDTA plasma (n=4) |
99 |
95-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.859 |
2.876 |
2.868 |
2.760 |
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| 20 |
2.261 |
2.234 |
2.248 |
2.140 |
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| 10 |
1.436 |
1.412 |
1.424 |
1.316 |
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| 5 |
0.744 |
0.723 |
0.734 |
0.626 |
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| 2.5 |
0.408 |
0.422 |
0.415 |
0.307 |
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| 1.25 |
0.251 |
0.237 |
0.244 |
0.136 |
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| 0.625 |
0.167 |
0.158 |
0.163 |
0.055 |
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| 0 |
0.106 |
0.110 |
0.108 |
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货期
3-5 working days