产品描述
IL-8(Interleukin-8),也称为中性粒细胞趋化因子1(Neutrophil chemotactic factor 1,NCF-1),是一种由多种细胞类型分泌的趋化因子,主要作用是吸引和激活白细胞(尤其是中性粒细胞)在炎症部位定向运动。IL-8通过结合两种不同的趋化因子受体CXCR1和CXCR2来介导其生物学效应。在机体免疫和炎症反应中,IL-8的表达水平显著升高,因此被广泛应用于炎症和免疫疾病的研究和诊断。此外,IL-8还参与了肿瘤的生长、转移和血管生成等生物学过程。
华美生物所提供的Dog Interleukin-8,IL-8 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测狗血清、血浆、细胞培养上清、组织匀浆样本中的IL8,其灵敏度为0.146 ng/mL,检测范围为0.312 ng/mL-20 ng/mL。
别名
CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
缩写
CXCL8
Uniprot No
P41324
灵敏度
0.146 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:2 |
Average % |
99 |
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| Range % |
95-105 |
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| 1:4 |
Average % |
88 |
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| Range % |
85-93 |
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| 1:8 |
Average % |
90 |
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| Range % |
86-97 |
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回收率
| The recovery of dog IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
86-94 |
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| EDTA plasma (n=4) |
88 |
85-95 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
3.221 |
3.295 |
3.258 |
3.157 |
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| 10 |
2.523 |
2.711 |
2.617 |
2.516 |
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| 5 |
1.791 |
1.930 |
1.861 |
1.760 |
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| 2.5 |
1.035 |
1.045 |
1.040 |
0.939 |
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| 1.25 |
0.466 |
0.485 |
0.476 |
0.375 |
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| 0.625 |
0.249 |
0.259 |
0.254 |
0.153 |
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| 0.312 |
0.157 |
0.167 |
0.162 |
0.061 |
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| 0 |
0.103 |
0.099 |
0.101 |
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货期
3-5 working days