产品描述

This Human HDAC9 ELISA Kit was designed for the quantitative measurement of Human HDAC9 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

HD 7 ELISA Kit; HD 7B ELISA Kit; HD 9 ELISA Kit; HD7 ELISA Kit; HD7B ELISA Kit; HD9 ELISA Kit; HDAC 7 ELISA Kit; HDAC 7B ELISA Kit; HDAC 9 ELISA Kit; HDAC 9B ELISA Kit; HDAC 9FL ELISA Kit; HDAC ELISA Kit; HDAC7 ELISA Kit; HDAC7B ELISA Kit; HDAC9 ELISA Kit; HDAC9_HUMAN ELISA Kit; HDAC9B ELISA Kit; HDAC9FL ELISA Kit; HDRP ELISA Kit; Histone deacetylase 4/5 related protein ELISA Kit; Histone deacetylase 7 ELISA Kit; Histone deacetylase 7B ELISA Kit; Histone deacetylase 9 ELISA Kit; Histone deacetylase 9A ELISA Kit; Histone deacetylase related protein ELISA Kit; Histone deacetylase-related protein ELISA Kit; KIAA0744 ELISA Kit; MEF2 interacting transcription repressor MITR ELISA Kit; MEF2 interacting transcription repressor protein ELISA Kit; MEF2-interacting transcription repressor MITR ELISA Kit; MITR ELISA Kit

缩写

HDAC9

Uniprot No

Q9UKV0

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human HDAC9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		85-94
1:2		Average %		96
		Range %		92-105
1:4		Average %		102
		Range %		95-109
1:8		Average %		88
		Range %		86-92

回收率

The recovery of human HDAC9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		86-94
EDTA plasma (n=4)		99		95-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.311 2.422 2.367 2.226 5 1.902 1.899 1.901 1.760 2.5 1.323 1.425 1.374 1.233 1.25 0.965 1.024 0.995 0.854 0.625 0.658 0.633 0.646 0.505 0.312 0.344 0.372 0.358 0.217 0.156 0.257 0.266 0.262 0.121 0 0.135 0.146 0.141

货期

3-5 working days