产品描述
IGF1 (Insulin-like growth factor 1)是一种肽类生长因子,其生物学功能主要是促进组织的增殖和修复。IGF1通过与细胞表面的受体结合,激活多条信号通路,如PI3K/Akt和ERK1/2通路,调节细胞增殖、分化和凋亡等生物学过程。IGF1含量受多种因素影响,如生长激素、胰岛素、营养状况和运动等。IGF1在胚胎发育、儿童生长、成人代谢调节、肌肉和骨骼维护等方面发挥着重要的生理作用。
华美生物所提供的Bovine Insulin-like growth factor 1,IGF-1 ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测牛血清、血浆、组织匀浆样本中的IGF1,其灵敏度为0.94 ng/mL,检测范围为0.75 ng/mL-60 ng/mL。
别名
IGF1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
缩写
IGF1
Uniprot No
P07455
灵敏度
0.94 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
86-94 |
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| 1:2 |
Average % |
96 |
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| Range % |
92-100 |
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| 1:4 |
Average % |
83 |
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| Range % |
80-86 |
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| 1:8 |
Average % |
91 |
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| Range % |
83-98 |
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回收率
| The recovery of bovine IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
87-99 |
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| EDTA plasma (n=4) |
93 |
91-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 60 |
0.387 |
0.389 |
0.388 |
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| 22.5 |
0.532 |
0.567 |
0.550 |
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| 11.25 |
0.764 |
0.789 |
0.777 |
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| 3.75 |
1.149 |
1.168 |
1.159 |
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| 0.75 |
1.636 |
1.638 |
1.637 |
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| 0 |
2.105 |
2.118 |
2.112 |
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货期
3-5 working days