产品描述
This Rabbit IFNA1 ELISA Kit was designed for the quantitative measurement of Rabbit IFNA1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.
别名
IFN-alpha,interferon alpha
缩写
IFN-α
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 82-92 | |
| 1:2 | Average % | 92 | |
| Range % | 88-98 | |
| 1:4 | Average % | 95 | |
| Range % | 90-100 | |
| 1:8 | Average % | 89 | |
| Range % | 86-94 | |
回收率
| The recovery of rabbit IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 84 | 80-88 | |
| EDTA plasma (n=4) | 94 | 88-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.853 | 2.681 | 2.767 | 2.675 | | | 200 | 2.224 | 2.331 | 2.278 | 2.186 | | | 100 | 1.498 | 1.541 | 1.520 | 1.428 | | | 50 | 0.876 | 0.887 | 0.882 | 0.790 | | | 25 | 0.443 | 0.432 | 0.438 | 0.346 | | | 12.5 | 0.239 | 0.227 | 0.233 | 0.141 | | | 6.25 | 0.150 | 0.146 | 0.148 | 0.056 | | | 0 | 0.092 | 0.091 | 0.092 | | |
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货期
3-5 working days