产品描述

This Human CYP2E1 ELISA Kit was designed for the quantitative measurement of Human CYP2E1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 43.75 pg/mL-2800 pg/mL and the sensitivity is 10.94 pg/mL.

别名

4-nitrophenol 2-hydroxylase ELISA Kit; CP2E1_HUMAN ELISA Kit; CPE1 ELISA Kit; CYP2E ELISA Kit; Cyp2e1 ELISA Kit; CYPIIE1 ELISA Kit; Cytochrome P450 2E1 ELISA Kit; Cytochrome P450 isozyme 3A ELISA Kit; Cytochrome P450 LM3A ELISA Kit; Cytochrome P450; family 2; subfamily e; polypeptide 1 ELISA Kit; cytochrome P450; subfamily IIE (ethanol-inducible); polypeptide 1 ELISA Kit; Cytochrome P450; subfamily IIE ELISA Kit; Cytochrome P450-ALC ELISA Kit; Cytochrome P450-J ELISA Kit; Cytochrome P450RLM6 ELISA Kit; EC 1.14.13.- ELISA Kit; EC 1.14.13.n7 ELISA Kit; Ethanol-inducible P450 ELISA Kit; Flavoprotein-linked monooxygenase ELISA Kit; MGC133529 ELISA Kit; Microsomal monooxygenase ELISA Kit; OTTHUMP00000020813 ELISA Kit; OTTHUMP00000046571 ELISA Kit; P450 ALC ELISA Kit; P450-J ELISA Kit; P450C2E ELISA Kit; P450J ELISA Kit; P450RLM6 ELISA Kit; Xenobiotic monooxygenase ELISA Kit

缩写

CYP2E1

Uniprot No

P05181

灵敏度

10.94 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CYP2E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		91-103
1:2		Average %		93
		Range %		85-98
1:4		Average %		99
		Range %		95-106
1:8		Average %		94
		Range %		90-98

回收率

The recovery of human CYP2E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		88-94
EDTA plasma (n=4)		97		95-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2800 1.830 1.952 1.891 1.791 1400 1.008 1.076 1.042 0.942 700 0.636 0.604 0.620 0.520 350 0.351 0.369 0.360 0.260 175 0.218 0.224 0.221 0.121 87.5 0.161 0.159 0.160 0.060 43.75 0.123 0.129 0.126 0.026 0 0.103 0.097 0.100

货期

3-5 working days