产品描述
人绒毛膜促性腺激素(Human Chorionic Gonadotropin,hCG)是一种由胎盘产生的激素,主要作用是促进胎儿生长和维持妊娠。除了在孕期检测中应用广泛之外,hCG的水平还可以作为某些疾病(如某些肿瘤)的生物标志物进行检测。此外,hCG还可以作为某些药物的指标,用于判断药物治疗的疗效。
华美生物所提供的Human Chorionic Gonadotrophin,HCG ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、尿液、细胞培养上清样本中的hCG,其灵敏度为2 mIU/mL,检测范围为8 mIU/mL-240 mIU/mL。
别名
CGB3 ELISA Kit; CGB; ELISA Kit; CGB5; ELISA Kit; CGB8 ELISA Kit; Choriogonadotropin subunit beta 3 ELISA Kit; Choriogonadotropin subunit beta ELISA Kit; CG-beta ELISA Kit; Chorionic gonadotropin chain beta ELISA Kit
缩写
hCG
Uniprot No
P0DN86
灵敏度
2 mIU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human HCG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
86-97 |
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| 1:2 |
Average % |
92 |
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| Range % |
85-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
92-100 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-98 |
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回收率
| The recovery of human HCG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
97 |
90-103 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mIU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 240 |
2.540 |
2.596 |
2.568 |
2.532 |
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| 100 |
1.279 |
1.299 |
1.289 |
1.253 |
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| 40 |
0.638 |
0.632 |
0.635 |
0.599 |
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| 16 |
0.285 |
0.291 |
0.288 |
0.252 |
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| 8 |
0.159 |
0.155 |
0.157 |
0.121 |
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| 0 |
0.036 |
0.035 |
0.036 |
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货期
3-5 working days