产品描述
This Rat HDL-C ELISA Kit was designed for the quantitative measurement of Rat HDL-C protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 93.75 ng/mL-6000 ng/mL and the sensitivity is 23.4 ng/mL.
缩写
HDL-C
灵敏度
23.4 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:200 | Average % | 87 | |
| Range % | 83-89 | |
| 1:400 | Average % | 99 | |
| Range % | 95-102 | |
| 1:800 | Average % | 90 | |
| Range % | 85-93 | |
| 1:1600 | Average % | 99 | |
| Range % | 92-102 | |
回收率
| The recovery of rat HDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-97 | |
| EDTA plasma (n=4) | 107 | 101-109 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 6000 | 2.612 | 2.554 | 2.583 | 2.483 | | | 3000 | 2.122 | 2.158 | 2.140 | 2.040 | | | 1500 | 1.449 | 1.453 | 1.451 | 1.351 | | | 750 | 0.845 | 0.831 | 0.838 | 0.738 | | | 375 | 0.420 | 0.434 | 0.427 | 0.327 | | | 187.5 | 0.272 | 0.278 | 0.275 | 0.175 | | | 93.75 | 0.176 | 0.169 | 0.173 | 0.073 | | | 0 | 0.101 | 0.099 | 0.100 | | |
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货期
3-5 working days