产品描述

This Human Coagulation factor V (F5) ELISA Kit was designed for the quantitative measurement of Human Coagulation factor V (F5) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

别名

Activated protein C cofactor ELISA Kit; APC cofactor ELISA Kit; coagulation factor V (proaccelerin; labile factor) ELISA Kit; Coagulation factor V ELISA Kit; coagulation factor V jinjiang A2 domain ELISA Kit; Coagulation factor V light chain ELISA Kit; F5 ELISA Kit; FA5_HUMAN ELISA Kit; Factor V Leiden ELISA Kit; FactorV ELISA Kit; FVL ELISA Kit; Labile factor ELISA Kit; PCCF ELISA Kit; Proaccelerin ELISA Kit; proaccelerin; labile factor ELISA Kit; Protein C cofactor ELISA Kit; RPRGL1 ELISA Kit; THPH2 ELISA Kit

缩写

F5

Uniprot No

P12259

灵敏度

0.78 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Blood Coagulation

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human FⅤ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1000		Average %		85
		Range %		80-89
1:2000		Average %		94
		Range %		90-98
1:4000		Average %		96
		Range %		92-100
1:8000		Average %		103
		Range %		99-107

回收率

The recovery of human FⅤ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		88		84-92
EDTA plasma (n=4)		93		89-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 200 2.444 2.536 2.490 2.360 100 2.186 2.099 2.143 2.013 50 1.754 1.682 1.718 1.588 25 1.156 1.104 1.130 1.000 12.5 0.668 0.715 0.692 0.562 6.25 0.403 0.387 0.395 0.265 3.12 0.289 0.284 0.287 0.157 0 0.132 0.128 0.130

货期

3-5 working days