产品描述
This Rat HSPB1 ELISA Kit was designed for the quantitative measurement of Rat HSPB1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.
别名
Hspb1 ELISA Kit; Hsp27 ELISA Kit; Heat shock protein beta-1 ELISA Kit; HspB1 ELISA Kit; Heat shock 27 kDa protein ELISA Kit; HSP 27 ELISA Kit
缩写
HSPB1
Uniprot No
P42930
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays):CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HSP-27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
93-102 |
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| 1:2 |
Average % |
105 |
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| Range % |
100-113 |
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| 1:4 |
Average % |
91 |
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| Range % |
85-96 |
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| 1:8 |
Average % |
94 |
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| Range % |
90-98 |
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回收率
| The recovery of rat HSP-27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
85-93 |
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| EDTA plasma (n=4) |
85 |
80-90 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.361 |
2.293 |
2.327 |
2.126 |
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| 25 |
1.674 |
1.592 |
1.633 |
1.432 |
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| 12.5 |
1.002 |
1.056 |
1.029 |
0.828 |
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| 6.25 |
0.729 |
0.704 |
0.717 |
0.516 |
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| 3.12 |
0.447 |
0.471 |
0.459 |
0.258 |
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| 1.56 |
0.335 |
0.318 |
0.327 |
0.126 |
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| 0.78 |
0.278 |
0.269 |
0.274 |
0.073 |
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| 0 |
0.196 |
0.205 |
0.201 |
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货期
3-5 working days